Question 1

Which PCR step causes the denaturation of double-stranded DNA?

Accepted Answer

A)   Add DNA polymerase and nucleotides at 72°C.
B)   Cool DNA to between 50°C and 65°C.
C)   Add primers.
D)   Heat target DNA to 94°C.
E)   Repeat the cycle of heating and cooling.F) All of the above
G) B) and E)

Question 2

The Western Blot technique detects

Accepted Answer

A)   DNA.
B)   RNA.
C)   proteins.
D)   recombinant DNA.
E)   specific genetic marker sequence on genes.F) B) and E)
G) D) and E)

Question 3

An example of gene therapy is the insertion of the gene for human growth hormone into
E. coli cells.

Accepted Answer

A) True 
 B)False

Question 4

The human gene for a protein hormone has been isolated. Scientists want to introduce this gene into a bacterial host, modifying the bacteria so that it can be used to produce this hormone. After successfully introducing the gene on a plid, the bacteria produced the protein hormone, but the protein was defective, longer than normal, with intervening stretches of amino acids not found in the native protein. Which solution would be most likely to resolve this problem?

Accepted Answer

A)   Treat the defective protein with enzymes.
B)   Introduce human ribosomes into the bacteria.
C)   Start over, introducing a complementary DNA cDN
A)   copy of the gene into the bacterial host.
D)   Introduce human mRNAs into the bacterial host.E) B) and C)
F) A) and D)

Question 5

Reverse transcriptase is used to make cDNA from an RNA template.

Accepted Answer

A) True 
 B)False

Question 6

The human gene for a protein hormone has been isolated. Scientists want to introduce this gene into a bacterial host, modifying the bacteria so that it can be used to produce this hormone. After successfully introducing the gene on a plid, the bacteria produced the protein hormone, but the protein was defective, longer than normal, with intervening stretches of amino acids not found in the native protein. What is the most likely explanation?

Accepted Answer

A)   The bacteria can't transcribe the human gene.
B)   The gene that was introduced contained introns.
C)   Bacterial cells cannot translate mRNAs transcribed from human genes.
D)   Bacterial ribosomes make errors when transcribing human mRNAs.E) A) and B)
F) A) and C)

Question 7

Both DNA sequencing and polymerase chain reactions require special __________ to initiate synthesis of a new DNA molecule.

Accepted Answer

A)   RNA promoters
B)   fluorescent nucleotides
C)   primers
D)   thermophilic polymerases
E)   endonucleasesF) B) and D)
G) C) and D)

Question 8

When trying to locate a mutation responsible for a disease, exome sequencing would provide an effective shortcut because

Accepted Answer

A)   it targets just protein-encoding sequences.
B)   it targets the sequencing or rRNA and tRNA encoding sequences of DNA.
C)   it provides the entire genome sequence.
D)   it targets the sequencing of mitochondrial DNA.E) All of the above
F) A) and B)

Question 9

Which of the following regions of DNA would be of most value for forensic analysis?

Accepted Answer

A)   DNA sequences that are typically identical for all humans
B)   DNA sequences that differ among humans, with a maximum of two different alleles
C)   DNA sequences that are quite variable with multiple different alleles
D)   Most DNA sequences are equally valuable for forensic analysis.E) B) and C)
F) None of the above

Question 10

Sequencing a gene automatically identifies which gene it is.

Accepted Answer

A) True 
 B)False

Question 11

DNA strands can be clipped crosswise at selected positions by using enzymes called

Accepted Answer

A)   palindromes.
B)   reverse transcriptases.
C)   restriction endonucleases.
D)   ligases.
E)   DNA polymerases.F) B) and D)
G) C) and E)

Question 12

The two common vectors used to transfer a piece of DNA into a cloning host are _____ and _____.

Accepted Answer

The answer of The two common vectors used to transfer...

Question 13

The function of the dideoxy dd)  nucleotides that are used in the Sanger method of DNA sequencing is to

Accepted Answer

A)   denature DNA into single strands.
B)   serve as primers.
C)   be a fluorescent tag.
D)   incorporate into newly replicated DNA strands and stop elongation.E) A) and D)
F) A) and C)

Question 14

Amplification of DNA is accomplished by

Accepted Answer

A)   polymerase chain reaction.
B)   DNA sequencing.
C)   gene probes.
D)   Southern blot.
E)   All of these choices are correct.F) B) and E)
G) B) and C)

Question 15

In recombinant DNA technique, an enzyme called _____ is needed to seal the sticky ends of genes into plids or chromosomes.

Accepted Answer

The answer of In recombinant DNA technique, an enzyme called...

Question 16

The size of DNA is often given in the number of _____ that it contains.

Accepted Answer

base pairs...

Question 17

The polymerase chain reaction PCR)  has been used in all of the following fields, except

Accepted Answer

A)   forensics.
B)   evolutionary studies.
C)   gene mapping.
D)   medicine.
E)   None of the choices; it is useful for all these fields.F) All of the above
G) D) and E)

Question 18

The various techniques by which scientists manipulate DNA in the lab are called

Accepted Answer

A)   genetic engineering.
B)   biotechnology.
C)   recombinant DNA.
D)   gel electrophoresis.
E)   gene probes.F) A) and B)
G) B) and C)

Question 19

The primers in PCR are

Accepted Answer

A)   synthetic DNA oligonucleotides.
B)   bacterial enzymes.
C)   short RNA strands.
D)   DNA polymerases.
E)   reverse transcriptases.F) B) and E)
G) A) and B)

Question 20

The use of an organism's biochemical processes to create a product is

Accepted Answer

A)   bioremediation.
B)   biotechnology.
C)   recombinant DNA.
D)   gel electrophoresis.
E)   gene probes.F) A) and B)
G) D) and E)

Exam 10: Genetic Engineering: a Revolution in Molecular Biology

Sequencing a gene automatically identifies which gene it is.

Correct Answer
verified

Which PCR step causes the denaturation of double-stranded DNA?

Correct Answer
verified

Which of the following regions of DNA would be of most value for forensic analysis?

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

The primers in PCR are

Correct Answer
verified

The function of the dideoxy dd) nucleotides that are used in the Sanger method of DNA sequencing is to

Correct Answer
verified

DNA strands can be clipped crosswise at selected positions by using enzymes called

Correct Answer
verified

In recombinant DNA technique, an enzyme called _____ is needed to seal the sticky ends of genes into plids or chromosomes.

Correct Answer
verified

Amplification of DNA is accomplished by

Correct Answer
verified

The polymerase chain reaction PCR) has been used in all of the following fields, except

Correct Answer
verified

When trying to locate a mutation responsible for a disease, exome sequencing would provide an effective shortcut because

Correct Answer
verified

The various techniques by which scientists manipulate DNA in the lab are called

Correct Answer
verified

Both DNA sequencing and polymerase chain reactions require special __________ to initiate synthesis of a new DNA molecule.

Correct Answer
verified

The Western Blot technique detects

Correct Answer
verified

The two common vectors used to transfer a piece of DNA into a cloning host are _ and _.

Correct Answer
verified

An example of gene therapy is the insertion of the gene for human growth hormone into E. coli cells.

Correct Answer
verified

The use of an organism's biochemical processes to create a product is

Correct Answer
verified

The size of DNA is often given in the number of _____ that it contains.

Correct Answer
verified
base pairs...
View Answer

Reverse transcriptase is used to make cDNA from an RNA template.

Correct Answer
verified

Exam 10: Genetic Engineering: a Revolution in Molecular Biology

Sequencing a gene automatically identifies which gene it is.

Correct AnswerverifiedShow Answer

Which PCR step causes the denaturation of double-stranded DNA?

Correct AnswerverifiedShow Answer

Which of the following regions of DNA would be of most value for forensic analysis?

Correct AnswerverifiedShow Answer

Correct AnswerverifiedShow Answer

Correct AnswerverifiedShow Answer

The primers in PCR are

Correct AnswerverifiedShow Answer

The function of the dideoxy dd) nucleotides that are used in the Sanger method of DNA sequencing is to

Correct AnswerverifiedShow Answer

DNA strands can be clipped crosswise at selected positions by using enzymes called

Correct AnswerverifiedShow Answer

In recombinant DNA technique, an enzyme called _____ is needed to seal the sticky ends of genes into plids or chromosomes.

Correct AnswerverifiedShow Answer

Amplification of DNA is accomplished by

Correct AnswerverifiedShow Answer

The polymerase chain reaction PCR) has been used in all of the following fields, except

Correct AnswerverifiedShow Answer

When trying to locate a mutation responsible for a disease, exome sequencing would provide an effective shortcut because

Correct AnswerverifiedShow Answer

The various techniques by which scientists manipulate DNA in the lab are called

Correct AnswerverifiedShow Answer

Both DNA sequencing and polymerase chain reactions require special __________ to initiate synthesis of a new DNA molecule.

Correct AnswerverifiedShow Answer

The Western Blot technique detects

Correct AnswerverifiedShow Answer

The two common vectors used to transfer a piece of DNA into a cloning host are _____ and _____.

Correct AnswerverifiedShow Answer

An example of gene therapy is the insertion of the gene for human growth hormone into E. coli cells.

Correct AnswerverifiedShow Answer

The use of an organism's biochemical processes to create a product is

Correct AnswerverifiedShow Answer

The size of DNA is often given in the number of _____ that it contains.

Correct Answerverifiedbase pairs...View AnswerShow Answer

Reverse transcriptase is used to make cDNA from an RNA template.

Correct AnswerverifiedShow Answer

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

The two common vectors used to transfer a piece of DNA into a cloning host are _ and _.

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified
base pairs...
View Answer

Correct Answer
verified