Question 1

You have made a specific DNA probe that will bind to a key sequence on the DNA of Neisseria gonorrhoeae,allowing your company to market a gonorrhea test kit that can be used to identify the bacterium in genital tract specimens.However,upon testing it out against a known Neisseria gonorrhoeae culture,you find that it does not work.Which of the following is a possible explanation for this negative result?

Accepted Answer

A)  You forgot to label the probe sequence with a reporter molecule.
B)  You forgot to digest the probe with restriction endonucleases.
C)  You forgot to add the mRNA to the DNA probe sample.
D)  You forgot to incubate the test at the 75C temperature required for hybridization.E) A) and D)
F) A) and C)

Question 2

Which of the following is not true of vectors?

Accepted Answer

A)  an origin of replication (ORI) is present.
B)  must accept DNA of desired size
C)  contain a gene for drug resistance
D)  easy to manipulate
E)  can detect RNA in cellsF) A) and E)
G) A) and B)

Question 3

Making new genomes is called

Accepted Answer

A)  bioengineering.
B)  synthetic biology.
C)  genetic engineering.
D)  cloning.
E)  recombinant DNA.F) B) and C)
G) B) and D)

Question 4

Two different nucleic acids can _____ by uniting at their complementary sites.

Accepted Answer

A)  hybridize
B)  covalently bond
C)  form a peptide bond
D)  ligateE) B) and C)
F) A) and D)

Question 5

DNA strands can be clipped crosswise at selected positions by using enzymes called

Accepted Answer

A)  palindromes.
B)  reverse transcriptases.
C)  restriction endonucleases.
D)  ligases.
E)  DNA polymerases.F) A) and D)
G) D) and E)

Question 6

Sequences of DNA that are identical when read from the 5' to 3' direction on one strand and the 3' to 5' direction on the other strand are

Accepted Answer

A)  palindromes.
B)  reverse transcriptases.
C)  restriction endonucleases.
D)  ligases.
E)  DNA polymerases.F) B) and E)
G) A) and E)

Question 7

The use of an organism's biochemical processes to create a product is referred to as

Accepted Answer

A)  genetic engineering.
B)  biotechnology.
C)  recombinant DNA.
D)  gel electrophoresis.
E)  gene probes.F) A) and B)
G) A) and C)

Question 8

The primers in PCR are

Accepted Answer

A)  synthetic DNA oligonucleotides.
B)  bacterial enzymes.
C)  short RNA strands.
D)  DNA polymerases.
E)  reverse transcriptases.F) B) and E)
G) A) and B)

Question 9

Common vectors used to transfer a piece of DNA into a cloning host are

Accepted Answer

A)  plasmids.
B)  viruses.
C)  bacteriophages.
D)  artificial chromosomes.
E)  All of the choices are correct.F) B) and C)
G) None of the above

Question 10

Genomic _____ are collections of isolated genes maintained in a cloning host.

Accepted Answer

A)  DNA
B)  libraries
C)  clones
D)  digests
E)  booksF) B) and D)
G) A) and E)

Question 11

What is the evolutionary advantage of bacteria producing restriction endonucleases?

Accepted Answer

A)  They make these enzymes for humans to use in manipulating DNA.
B)  Bacteria use these enzymes to repair their own mistakes made during DNA replication.
C)  Bacteria use these enzymes to attack other bacteria and destroy their DNA.
D)  These enzymes are a defensive measure of bacteria to defend themselves against invading DNA of bacteriophages.E) B) and D)
F) A) and D)

Question 12

The process of introducing a needed,normal gene,into human cells is called DNA mapping.

Accepted Answer

A) True 
 B)False

Question 13

What type of DNA map gives locations and sizes of DNA sections?

Accepted Answer

A)  linkage
B)  sequence
C)  physical
D)  geographical
E)  chromosomalF) A) and D)
G) None of the above

Question 14

Humans display how much DNA similarity with mice?

Accepted Answer

A)  50%
B)  60%
C)  70%
D)  80%
E)  90%F) A) and D)
G) B) and E)

Question 15

Genetically modified organisms include:

Accepted Answer

A)  bacteria.
B)  viruses.
C)  plants.
D)  nonhuman animals.
E)  All of the above have been genetically modifiedF) C) and D)
G) A) and B)

Question 16

What type of DNA map is most detailed?

Accepted Answer

A)  linkage
B)  sequence
C)  physical
D)  geographical
E)  chromosomalF) A) and C)
G) A) and B)

Question 17

Recombinant strains of this organism are released to colonize plant roots to produce an insecticide to destroy invading insects:

Accepted Answer

A)  Escherichia coli
B)  Saccharomyces cerevisiae
C)  Thermus aquaticus
D)  Pseudomonas syringae
E)  Pseudomonas fluorescensF) A) and E)
G) A) and D)

Question 18

The study of genomes of a particular community is called

Accepted Answer

A)  metagenomics.
B)  genomics.
C)  proteomics.
D)  genomology.
E)  metabolomics.F) C) and D)
G) All of the above

Question 19

Transgenic organisms are

Accepted Answer

A)  created in nature.
B)  only microorganisms.
C)  copyrighted.
D)  patented.E) A) and B)
F) A) and C)

Question 20

You want to identify Mycobacterium tuberculosis in a patient sputum sample.Which procedure would be most useful in this case?

Accepted Answer

A)  polymerase chain reaction
B)  whole genome sequencing
C)  DNA probe analysis
D)  microarray analysisE) A) and B)
F) A) and C)

Exam 10: Genetic Engineering and Recombinant Dna

What type of DNA map gives locations and sizes of DNA sections?

Correct Answer
verified

The process of introducing a needed,normal gene,into human cells is called DNA mapping.

Correct Answer
verified

What is the evolutionary advantage of bacteria producing restriction endonucleases?

Correct Answer
verified

Common vectors used to transfer a piece of DNA into a cloning host are

Correct Answer
verified

The study of genomes of a particular community is called

Correct Answer
verified

Making new genomes is called

Correct Answer
verified

Sequences of DNA that are identical when read from the 5' to 3' direction on one strand and the 3' to 5' direction on the other strand are

Correct Answer
verified

Transgenic organisms are

Correct Answer
verified

The primers in PCR are

Correct Answer
verified

You want to identify Mycobacterium tuberculosis in a patient sputum sample.Which procedure would be most useful in this case?

Correct Answer
verified

The use of an organism's biochemical processes to create a product is referred to as

Correct Answer
verified

DNA strands can be clipped crosswise at selected positions by using enzymes called

Correct Answer
verified

Humans display how much DNA similarity with mice?

Correct Answer
verified

Correct Answer
verified

Two different nucleic acids can _____ by uniting at their complementary sites.

Correct Answer
verified

What type of DNA map is most detailed?

Correct Answer
verified

Genomic _____ are collections of isolated genes maintained in a cloning host.

Correct Answer
verified

Which of the following is not true of vectors?

Correct Answer
verified

Recombinant strains of this organism are released to colonize plant roots to produce an insecticide to destroy invading insects:

Correct Answer
verified

Genetically modified organisms include:

Correct Answer
verified

Exam 10: Genetic Engineering and Recombinant Dna

What type of DNA map gives locations and sizes of DNA sections?

Correct AnswerverifiedShow Answer

The process of introducing a needed,normal gene,into human cells is called DNA mapping.

Correct AnswerverifiedShow Answer

What is the evolutionary advantage of bacteria producing restriction endonucleases?

Correct AnswerverifiedShow Answer

Common vectors used to transfer a piece of DNA into a cloning host are

Correct AnswerverifiedShow Answer

The study of genomes of a particular community is called

Correct AnswerverifiedShow Answer

Making new genomes is called

Correct AnswerverifiedShow Answer

Sequences of DNA that are identical when read from the 5' to 3' direction on one strand and the 3' to 5' direction on the other strand are

Correct AnswerverifiedShow Answer

Transgenic organisms are

Correct AnswerverifiedShow Answer

The primers in PCR are

Correct AnswerverifiedShow Answer

You want to identify Mycobacterium tuberculosis in a patient sputum sample.Which procedure would be most useful in this case?

Correct AnswerverifiedShow Answer

The use of an organism's biochemical processes to create a product is referred to as

Correct AnswerverifiedShow Answer

DNA strands can be clipped crosswise at selected positions by using enzymes called

Correct AnswerverifiedShow Answer

Humans display how much DNA similarity with mice?

Correct AnswerverifiedShow Answer

Correct AnswerverifiedShow Answer

Two different nucleic acids can _____ by uniting at their complementary sites.

Correct AnswerverifiedShow Answer

What type of DNA map is most detailed?

Correct AnswerverifiedShow Answer

Genomic _____ are collections of isolated genes maintained in a cloning host.

Correct AnswerverifiedShow Answer

Which of the following is not true of vectors?

Correct AnswerverifiedShow Answer

Recombinant strains of this organism are released to colonize plant roots to produce an insecticide to destroy invading insects:

Correct AnswerverifiedShow Answer

Genetically modified organisms include:

Correct AnswerverifiedShow Answer

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